Quantitative Detection of t(14;18)-Positive Cells by Real-Time Quantitative PCR Using Fluorogenic Probes
نویسندگان
چکیده
منابع مشابه
Quantitative detection of t(14;18)-positive cells by real-time quantitative PCR using fluorogenic probes.
To detect t(14;18)-positive cells present in human lymphoma tissue, bone marrow aspirates and peripheral blood mononuclear cells (PBMNC), we have established an automated, real-time quantitative PCR using double-labeled fluorogenic probes. In relation to t(14;18)-positive genomic DNA or a cloned t(14;18)-DNA fragment, highly reproducible results can be obtained with initial copy numbers between...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98256cr05